Composite

Part:BBa_K3317030

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-16)


Converts vanillic acidcis to muconic acid

operon corresponding to the production of Vanillate O-demethylase (BBa_K3317000)under the vanillinic acid sensitive pVanCC promoter (BBa_K3317006), also under the control of the protocatechuic acid sensitive p3B5C promoter (BBa_K3317007) (PCB) produces the enzymes Protocatechuate decarboxylase (BBa_K3317002) and Catechol 1,2-dioxygenase (BBa_K3317003) carrying a signal peptide to be introduced into bacterial microcompatments (BMC)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1512
    Illegal BglII site found at 4298
    Illegal BamHI site found at 4
    Illegal BamHI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 465
    Illegal NgoMIV site found at 1246
    Illegal NgoMIV site found at 3573
    Illegal NgoMIV site found at 3645
    Illegal AgeI site found at 1733
    Illegal AgeI site found at 2059
    Illegal AgeI site found at 3345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1468
    Illegal SapI.rc site found at 2723
    Illegal SapI.rc site found at 4257


[edit]
Categories
Parameters
None